⚗️Henderson-Hasselbalch Calculator
The Henderson-Hasselbalch equation calculates the pH of a buffer solution containing a weak acid and its conjugate base. pH = pKa + log([A⁻]/[HA]). It is fundamental in biochemistry for maintaining physiological pH.
- 1Identify the weak acid (HA) and its conjugate base (A⁻)
- 2Find or calculate pKa = −log(Ka)
- 3Measure concentrations [HA] and [A⁻]
- 4pH = pKa + log([A⁻]/[HA])
Acetic acid pKa=4.76 · [CH₃COO⁻]=0.1M · [CH₃COOH]=0.1M=pH = 4.76Equal concentrations → log(1)=0 → pH=pKa
Buffer capacity
Buffers work best when pH is within ±1 of pKa. Outside this range, the buffer loses effectiveness because one of the acid/base pair becomes negligible.
⭐
Fun Fact
Blood is a bicarbonate buffer system (pKa 6.1) maintained at pH 7.4. Deviations of ±0.4 units cause acidosis or alkalosis — life-threatening conditions.
References
🔒
100% Gratis
Sin registro
✓
Preciso
Fórmulas verificadas
⚡
Instantáneo
Resultados al instante
📱
Compatible móvil
Todos los dispositivos