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Cara Menghitung Mass Spec

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Mass spectrometry measures mass-to-charge ratios (m/z) of ions; determines molecular weight and fragment composition.

Panduan Langkah demi Langkah

  1. 1Input mass spectrum peaks and intensities
  2. 2Identify molecular ion, fragments, and base peak
  3. 3Determine molecular structure

Contoh Terpecahkan

Masukan
Molecular ion m/z = 88, base peak m/z = 73 (M-15, loss of CH₃)
Hasil
Suggests methyl group loss

Kesalahan Umum yang Harus Dihindari

  • Misidentifying molecular ion (not always M⁺)
  • Not accounting for isotope patterns

Pertanyaan yang sering diajukan

What's base peak?

Most intense peak; usually stable fragment ion; useful for structure determination.

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