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Mass spectrometry measures mass-to-charge ratios (m/z) of ions; determines molecular weight and fragment composition.
ଷ୍ଟେପ୍-ଷ୍ଟେପ୍ ଗାଇଡ୍ |
- 1Input mass spectrum peaks and intensities
- 2Identify molecular ion, fragments, and base peak
- 3Determine molecular structure
ସମାଧାନ ହୋଇଥିବା ଉଦାହରଣ
ଇନପୁଟ୍
Molecular ion m/z = 88, base peak m/z = 73 (M-15, loss of CH₃)
ଫଳ
Suggests methyl group loss
ଏଡ଼ାଇବା ଯୋଗ୍ୟ ସାଧାରଣ ଭୁଲ
- ✕Misidentifying molecular ion (not always M⁺)
- ✕Not accounting for isotope patterns
ବାରମ୍ବାର ଜିଜ୍ଞାସା
What's base peak?
Most intense peak; usually stable fragment ion; useful for structure determination.
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