Jinsi ya kukokotoa Mass Spec
Mass Spec ni nini?
Mass spectrometry measures mass-to-charge ratios (m/z) of ions; determines molecular weight and fragment composition.
Mwongozo wa Hatua kwa Hatua
- 1Input mass spectrum peaks and intensities
- 2Identify molecular ion, fragments, and base peak
- 3Determine molecular structure
Mifano Iliyotatuliwa
Ingizo
Molecular ion m/z = 88, base peak m/z = 73 (M-15, loss of CH₃)
Matokeo
Suggests methyl group loss
Makosa ya Kawaida ya Kuepuka
- ✕Misidentifying molecular ion (not always M⁺)
- ✕Not accounting for isotope patterns
Maswali yanayoulizwa mara kwa mara
What's base peak?
Most intense peak; usually stable fragment ion; useful for structure determination.
Je, uko tayari kukokotoa? Jaribu Kikokotoo kisicholipishwa cha Mass Spec
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